Topoisomerase II Inhibitor; BNS-22

General description

A potent, 2H-chromen-2-one small molecule inhibitor that is selective for TOP2 (IC₅₀ = 2.8 µM and 0.42 µM for TOP2α and TOP2β, respectively). Unlike TOP2 poisons such as etoposide, it does not lead to DNA damage associated with DNA Double-Strand Breaks, as evidenced by the lack of γ-H2AX accumulation but instead inhibits kinetoplast DNA decatenation in vitro. This compound affects cell cycle at the M phase and disturbs mitotic spindle formation in HeLa cells at 3-10 µM. It also exhibits significant anti-proliferative activities against several human cancer cell lines.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kawatani, M., et al. 2011. Chem Biol.18, 743.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)